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- In the cosmetics industry, ATDNs are used as UV filters to protect skin from harmful UV radiation. Their small size allows them to be easily incorporated into various cosmetic products, providing effective sun protection without the typical side effects associated with chemical sunscreens. In addition, ATDNs also exhibit excellent photostability, ensuring long-lasting protection against UV rays.
- The primary function of Anatase Titanium Dioxide in food is as a coloring agent. Its intense whiteness can effectively brighten and whiten foods such as powdered sugar, confectionery, and dairy products. By adding small amounts of this compound, manufacturers can achieve a more visually appealing product that attracts consumers. Moreover, its high refractive index means that it can be used to add a glossy finish to certain foods, further enhancing their appeal.
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It's also added directly to food; mainly for coloring, but also as a thickener and to keep some powdered food, like confectioner's sugar, from clumping.


The most common foods containing titanium dioxide are chewing gum, candies, pastries, chocolates, coffee creamers, and cake decorations (1Trusted Source, 3Trusted Source).
Biointerfaces, Biomimicking, and Biohybrid Systems
The ingredient in question? Titanium dioxide.
When manufacturers add titanium dioxide to foods and other ingestible products, it’s typically referred to as E171, which relates to food-grade purity.
States looking to ban titanium dioxide as a food additive
Studies of titanium dioxide as a food additive suggest health dangers
In a study published in the journal Food and Chemical Toxicology in 2016, researchers investigated whether titanium dioxide exposure led to an increase in colorectal tumor creation in mice by using a colitis associated cancer model. By measuring tumor progression markers, the researchers found that mice given titanium dioxide experienced enhanced tumor formation in the distal colon. There was also a decrease of cells that act as a protective barrier in the colon. The researchers wrote: “These results suggest that E171 could worsen pre-existent intestinal diseases.”
This constant high rate of ROS production leads rapidly to extreme macromolecular oxidation, here it is observed in the AOPP and MDA detected after 3 h in samples treated with bare P25TiO2NPs (Fig. 6, Fig. 7). Macromolecular oxidation includes, among others, both protein and lipid oxidation. The ROS causes protein oxidation by direct reaction or indirect reactions with secondary by-products of oxidative stress. Protein fragmentation or cross-linkages could be produced after the oxidation of amino acid side chains and protein backbones. These and later dityrosine-containing protein products formed during excessive production of oxidants are known as advanced oxidation protein products (AOPP). They absorb at 340 nm and are used to estimate the damage to structural cell amino acids. Lipid oxidation is detected by the conjugation of oxidized polyunsaturated lipids with thiobarbituric acid, forming a molecule that absorbs light at 532 nm. Polyunsaturated lipids are oxidized as a result of a free-radical-mediated chain of reactions. The most exposed targets are usually membrane lipids. The macromolecular damage could represent a deadly danger if it is too extensive, and this might be the case. Moreover, it could be observed that cellular damage continues further and becomes irrevocable after 6 h and MDA could not be detected. This may be due to the fact that the lipids were completely degraded and cells were no longer viable. Lipids from the cell membrane are the most prone to oxidation. In fact, lipid peroxidation biomarkers are used to screen the oxidative body balance [51]. At the same time, AOPP values are up to 30 times higher for bare nanoparticles in comparison to the functionalized ones.
A safety review conducted by the EFSA in 2021 assessed thousands of studies published on titanium dioxide.
Less frequently, we ingest E171 through liquids such as salad dressing, dairy products, and some artificially colored drinks. However, since E171 is insoluble, manufacturers must use other stabilizers to keep E171 suspended in liquids as an emulsion; otherwise, it will settle to the bottom.
Lithopone
The European Commission banned titanium dioxide as a food additive in the EU in 2022 after the European Food Safety Authority (EFSA) conducted an updated safety assessment of E171 and concluded the panel could not eliminate concerns about its genotoxicity.
Lithopone, a chemical compound with a rich history, emerges as a vital substance in various industries. Comprising barium sulfate and zinc sulfide, this compound boasts unique properties that make it a popular choice in applications such as paints, inks, and plastics. Recognized for its exceptional opacity and brightness, lithopone significantly enhances the covering power of materials in which it is incorporated. Its inert nature and resistance to atmospheric influences contribute to its longevity in diverse formulations. As a white pigment, lithopone plays a pivotal role in achieving vibrant and enduring colors across a spectrum of products, marking it as a cornerstone in the realm of chemical compounds.
Barium sulfide is produced by carbothermic reduction of barium sulfate. Zinc sulfate is obtained from a variety of zinc products, often waste, by treatment with sulfuric acid.
Technical Specifications:(Standard:Q/SNBJ1-2012)
Numerous studies have linked titanium dioxide to genotoxicity and cytotoxicity. Genotoxicity refers to a chemical’s potential to cause DNA damage, which can, in turn, lead to cancer. Cytotoxicity is a general term that refers to a characteristic of being harmful to cells.
In a study published in the journal Toxicology, researchers examined the effects of exposing human colon cancer cell line (HTC116) titanium dioxide food additives in vitro. “In the absence of cytotoxicity, E171 was accumulated in the cells after 24 hours of exposure, increasing granularity and reactive oxygen species, inducing alterations in the molecular pattern of nucleic acids and lipids, and causing nuclei enlargement, DNA damage and tubulin depolymerization,” the scientists wrote. Researchers removed the additive from the culture, then examined the results 48 hours later. They found, “The removal of E171 was unable to revert the alterations found after 24 h of exposure in colon cells. In conclusion, exposure to E171 causes alterations that cannot be reverted after 48 h if E171 is removed from colon cells.”
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